- What makes MacConkey agar selective?
- Is MacConkey Agar defined?
- What type of media is blood agar?
- What color is E coli on MacConkey Agar?
- What is the purpose of the MacConkey Agar?
- What is the main use of the EMB agar plate?
- What is the purpose of blood agar?
- What are the types of agar?
- What is the use of nutrient agar?
- Why is MacConkey agar used for E coli?
- Is E coli Gram positive or negative?
What makes MacConkey agar selective?
This medium is both selective and differential.
The selective ingredients are the bile salts and the dye, crystal violet which inhibit the growth of Gram-positive bacteria.
The differential ingredient is lactose..
Is MacConkey Agar defined?
MacConkey agar is an indicator, a selective and differential culture medium for bacteria designed to selectively isolate Gram-negative and enteric (normally found in the intestinal tract) bacilli and differentiate them based on lactose fermentation.
What type of media is blood agar?
Blood agar is an enriched, bacterial growth medium. Fastidious organisms, such as streptococci, do not grow well on ordinary growth media. Blood agar is a type of growth medium (trypticase soy agar enriched with 5% sheep blood) that encourages the growth of bacteria, such as streptococci, that otherwise wouldn’t grow.
What color is E coli on MacConkey Agar?
dark pinkPresumptive identification of E. coli was made based on its characteristic morphology colony on the selective medium MacConkey agar. Rapid lactose fermenting colonies of E. coli appear dry, donut shaped and dark pink in color and are surrounded with dark pink area of precipitated bile salts.
What is the purpose of the MacConkey Agar?
MacConkey Agar (MAC) is a selective and differential medium designed to isolate and differentiate enterics based on their ability to ferment lactose. Bile salts and crystal violet inhibit the growth of Gram positive organisms. Lactose provides a source of fermentable carbohydrate, allowing for differentiation.
What is the main use of the EMB agar plate?
EMB is a selective, differential agar medium used for isolation of gram negative rods in a variety of specimen types. It is used frequently in clinical laboratories.
What is the purpose of blood agar?
Many species of bacteria produce toxic by-products that are capable of destroying red blood cells. Blood agar is a general purpose enriched medium often used to grow fastidious organisms and to differentiate bacteria based on their hemolytic properties.
What are the types of agar?
Types of agar platesBlood agar – contains blood cells from an animal (e.g. a sheep). … Chocolate agar – this contains lysed blood cells, and is used for growing fastidious (fussy) respiratory bacteria.Neomycin agar – contains the antibiotic neomycin.Sabouraud agar – used for fungi.More items…
What is the use of nutrient agar?
Nutrient agar is a general purpose medium supporting growth of a wide range of non-fastidious organisms. It typically contains (mass/volume): 0.5% Peptone – this provides organic nitrogen. 0.3% beef extract/yeast extract – the water-soluble content of these contribute vitamins, carbohydrates, nitrogen, and salts.
Why is MacConkey agar used for E coli?
Sorbitol MacConkey agar is a variant of traditional MacConkey agar used in the detection of E. coli O157:H7. … This is important because gut bacteria, such as Escherichia coli, can typically ferment lactose, while important gut pathogens, such as Salmonella enterica and most shigellas are unable to ferment lactose.
Is E coli Gram positive or negative?
E coli is a gram-negative bacillus that grows well on commonly used media. It is lactose-fermenting and beta-hemolytic on blood agar. Most E coli strains are nonpigmented. The image below shows Escherichia coli on Gram staining.